Highly sensitive oxygen sensor for cell culture
AssigneeNational Institute of Standards and Technology, U.S. Department of Commerce
InventorsSamuel P. Forry, Peter C. Thomas
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Abstract
An oxygen sensor comprising an oxygen sensing compound and configured to substantially mitigate leaching of the oxygen sensing compound from the oxygen sensor to an outer surface thereof is provided. The oxygen sensor may comprise one or more layers. A first portion of the oxygen sensor is configured to be permeable to gas and comprises an oxygen sensing material. A second portion is disposed with or on the first portion and is configured to be permeable to gas and substantially impermeable to the oxygen sensing material.
Claims20
An oxygen sensor comprising:
- a first layer configured to be permeable to gas and comprising an oxygen sensing material;
- a second layer disposed adjacent said first layer configured to be permeable to gas and substantially impermeable to said oxygen sensing material;
- and
- a third layer disposed adjacent said second layer configured to be permeable to gas and facilitate cell attachment therewith.
An oxygen sensor comprising:
- a cytotoxic oxygen sensing material substantially homogenously dispersed within a substrate;
- said substrate having a permeability to oxygen of at least 500 Barrer;
- a barrier layer on said substrate;
- said barrier layer having a permeability to oxygen of at least 500 Barrer;
- and
- said barrier layer being configured to mitigate a cytotoxic effect of said cytotoxic oxygen sensing material on a cell culture by at least 90%, during an oxygen sensing period.
An oxygen sensor comprising a first portion and a second portion;
- said first portion comprising an oxygen sensing compound;
- said second portion being disposed on said first portion and configured to substantially mitigate leaching of said oxygen sensing compound from said first portion to an outer surface of said second portion;
- and
- said second portion being configured to have a permeability to oxygen of at least 500 Barrer.
Dependencies are inferred from the claim text.