Bacillus licheniformis chromosome
AssigneesNovozymes A/S, Novozymes, Inc.
InventorsRandy Berka, Michael Rey, Preethi Ramaiya, Jens Tønne Andersen, Michael Dolberg Rasmussen, Peter Bjarke Olsen
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Abstract
The present invention relates to an isolated polynucleotide of the complete chromosome of Bacillus licheniformis. The present invention also relates to isolated genes of the chromosome of Bacillus licheniformis which encode biologically active substances and to nucleic acid constructs, vectors, and host cells comprising the genes as well as methods for producing biologically active substances encoded by the genes and to methods of using the isolated genes of the complete chromosome of Bacillus licheniformis.
Claims9
An isolated polypeptide having biological activity, selected from the group consisting of:
- (a) a polypeptide having biological activity comprising an amino acid sequence having at least 90% identity with an amino acid sequence selected from the group consisting of SEQ ID NOs:
- 4199-8395;
- and
- (b) a polypeptide having biological activity encoded by a gene comprising a nucleotide sequence which hybridizes under medium-high stringency conditions with a nucleotide sequence selected from the group consisting of SEQ ID NOs:
- 2-4198, or the full-length complementary strand thereof, wherein high stringency conditions are defined as prehybridization and hybridization at 42′C in 5×SSPE, 0.3% SDS, 200 μg/ml sheared and denatured salmon sperm DNA, and 35% formamide, and washing three times each for 15 minutes using 2×SSC, 0.2% SDS 60° C.
Dependencies are inferred from the claim text.