Chimeric Polymerases
AssigneeLife Technologies Corporation
InventorsPATRICK K. MARTIN, David A. Simpson
Life of this patent
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Abstract
Disclosed herein are chimeric polymerases and methods of making and using same.
Claims27
A chimeric polypeptide comprising a polymerizing domain and a dUTPase domain.
5-12. (canceled)
15-22. (canceled)
27-28. (canceled)
A chimeric polypeptide comprising a type B polymerizing domain and a dUTPase domain, wherein said polymerizing domain is positioned amino terminal to said dUTPase domain and said chimeric polypeptide is thermostable.
The chimeric polypeptide of 29, which further comprises a non-specific DNA binding domain.
31-40. (canceled)
43-46. (canceled)
49-50. (canceled)
A chimeric polypeptide comprising at least a type B polymerizing domain with reduced base analog detection activity and a non-specific nucleic acid binding domain that is at least about 95% identical to the amino acid sequence of Pae3192 or Ape3192.
55-87. (canceled)
Dependencies are inferred from the claim text.