Method for producing hydroxylated adamantane using cytochrome p450
AssigneeShjonogi & Co., Ltd.
InventorsTaijiro Tomikawa, Yuuichi Mitsuda
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Abstract
Disclosed is a method for producing a hydroxylated form of a compound having an adamantane skeleton, which is useful as an intermediate for functional resins and pharmaceutical products, with high yield and at low cost. Specifically, a hydroxylated form of a compound having an adamantane skeleton can be obtained by using cytochrome P450. More specifically, an N-(5-hydroxy-2-adamantyl)-benzamide derivative can be produced by hydroxylating an N-(2-adamantyl)-benzamide derivative.
Claims15
A process for producing an N-(5-hydroxy-2-adamantyl)-benzamide derivative characterized by hydroxylating an N-(2-adamantyl)-benzamide derivative using cytochrome P450.
A method for hydroxylating an adamantane part of a compound having an adamantane skeleton, comprising using
- a protein consisting of an amino acid sequence of SEQ ID No.:
- 1 or 3, or
- a protein consisting of an amino acid sequence in which one to several amino acids are deleted, substituted or added in the amino acid sequence, and having hydroxylation activity.
A protein containing an amino acid sequence in which one to several amino acids are deleted, substituted or added in the amino acid sequence of SEQ ID No.:
- 1, and having hydroxylation activity.
A protein consisting of an amino acid sequence having one to several mutations selected from the group consisting of I77F, L357P, A362T, F41L, I77W, M105I, T234A, F232I, F232L and F232M in the amino acid sequence of SEQ ID No.:
- 1.
A protein consisting of the amino acid sequence of SEQ ID No.:
- 3, or
- a protein containing an amino acid sequence in which one to several amino acids are deleted, substituted or added in the amino acid sequence, and having hydroxylation activity.
A protein consisting of an amino acid sequence having one to several mutations selected from the group consisting of I77F, I77W, M105I, A196D, F232I, F232L, F232M, T234A, T244A, V399E and K702E in the amino acid sequence of SEQ ID No.:
- 3.
A compound of the formula 5:
- a salt or a solvate thereof.
A compound of the formula 6:
- a salt or solvate thereof,
- wherein R is hydrogen or a hydrophilic group.
Dependencies are inferred from the claim text.