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AssigneeKaneka Corporation
InventorsHiroyuki Kanamaru, Makoto Ueda, Ruuji Miki, Hirokazu Nanba
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The present invention relates to novel D-amino acid oxidase isolated and purified from Candida intermedia, a gene encoding the D-amino acid oxidase, a recombinant plasmid containing the gene, and a transformant into which the D-amino acid oxidase gene has been introduced, as well as a production method of D-amino acid oxidase including culturing the transformant. Moreover, the present invention relates to a production method of L-amino acids, 2-oxo acids or cyclic imines, which include reacting racemic amino acids with the D-amino acid oxidase, more preferably, a production method of L-amino acids, which includes reacting racemic amino acid with the D-amino acid oxidase, amino acid dehydrogenase and an enzyme having a coenzyme-regenerating activity. According to the present invention, L-amino acids, 2-oxo acids or cyclic imines can be produced with good efficiency in an industrial scale.
-70. (canceled)
A DNA of the following (a), (b), (c), (d), (e) or (f):
A polypeptide of the following (g), (h) or (i):
A polypeptide having D-amino acid oxidase activity derived from Candida intermedia and the following physicochemical properties:
A recombinant plasmid comprising, as inserts, a DNA encoding a polypeptide having a D-amino acid oxidase activity and a DNA encoding a polypeptide having an enzyme activity different from that of the D-amino acid oxidase, and affording an improved amount of the D-amino acid oxidase produced in a transformed host microorganism as compared to an amount of the D-amino acid oxidase produced in a host microorganism transformed with a recombinant plasmid comprising said DNA encoding the polypeptide having the D-amino acid oxidase activity alone as an insert.
A transformant obtained by transforming a host microorganism with a DNA encoding a polypeptide having a D-amino acid oxidase activity and a DNA encoding a polypeptide having an enzyme activity different from that of the D-amino acid oxidase, and showing an improved D-amino acid oxidase activity as compared to a transformant transformed with a DNA encoding the polypeptide having the D-amino acid oxidase activity alone.
Dependencies are inferred from the claim text.