Expression Vector
AssigneeFujifilm Diosynth Biotechnologies UK Limited
InventorsBhupendra Vallabh Kara, Christopher David John Lennon
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Abstract
An expression vector for expressing a target polypeptide in a prokaryotic cell is provided. The vector comprises a promoter operably linked to a polynucleotide encoding the target polypeptide operably linked to a eukaryotic secretion leader sequence, the eukaryotic secretion leader sequence encoding a signal peptide sequence selected from the group consisting of: a) MLKRSSWLATLGLLTVASVSTIVYA; b) MKKATFITCLLAVLLVSNPIWNA; c) MKVSAAALAVILIATALCAPASA; d) MKVSTAFLCLLLTVSAFSAQVLA; and e) MKCLLLALGLALACAAQA. Processes for expressing polypeptides and prokaryotic microorganisms comprising such vectors are also provided.
Claims15
An expression vector for expressing a target polypeptide in a prokaryotic cell, comprising a promoter operably linked to a polynucleotide encoding a target polypeptide operably linked to a eukaryotic secretion leader sequence, the eukaryotic secretion leader sequence encoding a signal peptide sequence selected from the group consisting of:
- a) MLKRSSWLATLGLLTVASVSTIVYA;
- (SEQ ID NO:
- 1)
- b) MKKATFITCLLAVLLVSNPIVVNA;
- (SEQ ID NO:
- 2)
- c) MKVSAAALAVILIATALCAPASA;
- (SEQ ID NO:
- 3)
- d) MKVSTAFLCLLLTVSAFSAQVLA;
- (SEQ ID NO:
- 4)
- and
- e) MKCLLLALGLALACAAQA
- (SEQ ID NO:
- 5)
- or a functional equivalent thereof.
A polynucleotide comprising a nucleotide sequence encoding a signal peptide sequence selected from the group consisting of:
- a) MLKRSSWLATLGLLTVASVSTIVYA;
- (SEQ ID NO:
- 1)
- b) MKKATFITCLLAVLLVSNPIVVNA;
- (SEQ ID NO:
- 2)
- c) MKVSAAALAVILIATALCAPASA;
- (SEQ ID NO:
- 3)
- d) MKVSTAFLCLLLTVSAFSAQVLA;
- (SEQ ID NO:
- 4)
- and
- e) MKCLLLALGLALACAAQA
- (SEQ ID NO:
- 5)
- or a functional equivalent thereof, operably linked to a nucleotide sequence encoding a recombinant polypeptide.
A polypeptide comprising a signal peptide sequence selected from the group consisting of:
- a) MLKRSSWLATLGLLTVASVSTIVYA;
- (SEQ ID NO:
- 1)
- b) MKKATFITCLLAVLLVSNPIVVNA;
- (SEQ ID NO:
- 2)
- c) MKVSAAALAVILIATALCAPASA;
- (SEQ ID NO:
- 3)
- d) MKVSTAFLCLLLTVSAFSAQVLA;
- (SEQ ID NO:
- 4)
- and
- e) MKCLLLALGLALACAAQA
- (SEQ ID NO:
- 5)
- or a functional equivalent thereof, operably linked to a recombinant target polypeptide.
12-13. (canceled)
Dependencies are inferred from the claim text.