Product and Process for Transformation of Thraustochytriales Microorganisms

A method for production of a protein, comprising:

• culturing a recombinant microorganism of the Order Thraustochytriales in a medium, wherein the recombinant microorganism comprises an isolated nucleic acid molecule comprising a foreign gene that encodes the protein, to produce the protein;
• and
• recovering the protein.

A method for production of a protein, comprising:

• culturing a recombinant microorganism of the Order Thraustochytriales in a medium, wherein the recombinant microorganism comprises an isolated nucleic acid molecule comprising a foreign gene that encodes the protein, to produce the protein;
• and
• harvesting the microorganism comprising the protein.

A method for transforming a microorganism cell, comprising:

• introducing into the microorganism cell of the Order Thraustochytriales a recombinant nucleic acid molecule comprising a nucleic acid sequence encoding a protein to be expressed and a selection marker;
• and
• selecting a microorganism cell that has been successfully transformed with the recombinant nucleic acid molecule.

A method for inactivating, deleting, or mutating a native gene of a microorganism, comprising:

• introducing into a microorganism cell of the Order Thraustochytriales a recombinant nucleic acid molecule comprising a nucleic acid sequence that hybridizes with a target nucleic acid sequence in the microorganism, wherein a gene in the microorganism is inactivated, deleted, or mutated by homologous recombination between the nucleic acid sequence that hybridizes and the target nucleic acid sequence;
• and
• selecting a microorganism cell comprising the inactivated, deleted, or mutated gene.