Chaperone-assisted protein expression and methods of use
AssigneeDuke University
InventorsDewey G. McCafferty, Amanda J. Hoertz
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Abstract
The present disclosure provides methods of utilizing chaperone proteins for the production of active protein such as those encoded by the genes of natural biosynthetic clusters. The methods provided herein have applicability for a wide variety of genes ranging from small fatty acid biosynthetic genes to large non-ribosomal peptide synthetase genes.
Claims23
A method for expressing a protein of interest, said method comprising co-expressing a nucleotide sequence encoding said protein of interest with at least one nucleotide sequence encoding a chaperonin protein in an expression system, to thereby express said protein of interest.
A composition comprising an isolated protein of interest, wherein said protein of interest is encoded by a gene of a biosynthetic cluster.
Dependencies are inferred from the claim text.