Cholesterol sensor
AssigneeRoche Diagnostics Operations, Inc.
InventorsEmma Naomi Kathleen WALLACE-DAVIS, Lindy Jane Murphy, Andrew William Allan
Life of this patent
Estimating the term…
Abstract
A method for the determination of the amount of cholesterol in a sample is provided. The method typically provides a breakdown of the HDL and LDL cholesterol contents of the sample.
Claims20
An electrochemical method for the determination of the amount of triglyceride in a sample, said method comprising reacting the sample with a second series of reagents comprising
- (a2) a surfactant comprising one or more bile acid derivatives or salts thereof;
- (b2) a triglyceride hydrolysing reagent;
- (c2) glycerol dehydrogenase;
- (d2) a coenzyme;
- and
- (e2) a redox agent capable of being oxidised or reduced to form a product;
- and electrochemically detecting the amount of product formed.
A reagent mixture for use in an electrochemical method for the determination of the amount of triglyceride in a sample, the reagent mixture comprising
- (a2) a surfactant comprising one or more bile acid derivatives or salts thereof;
- (b2) a triglyceride hydrolysing reagent;
- (c2) glycerol dehydrogenase;
- (d2) a coenzyme;
- and
- (e2) a redox agent capable of being oxidised or reduced to form a product.
A kit for measuring the triglyceride content of a sample, the kit comprising
- one or more electrochemical cells, each cell having a working electrode, a reference or pseudo reference electrode and optionally a separate counter electrode;
- a second series of reagents comprising:
- (a2) a surfactant comprising one or more bile acid derivatives or salts thereof;
- (b2) a triglyceride hydrolysing reagent;
- (c2) glycerol dehydrogenase;
- (d2) a coenzyme;
- and
- (e2) a redox agent capable of being oxidised or reduced to form a product, said second series of reagents being associated with one of said electrochemical cells;
- a power supply for applying a potential across each cell;
- and
- a measuring instrument for measuring the resulting electrochemical response of each cell.
A method of breaking down the lipoprotein structure of a sample comprising lipoproteins by using one or more bile acid derivatives or salts thereof as a surfactant, in an electrochemical method for the determination of the amount of triglyceride in a sample, said method comprising reacting the sample with a second series of reagents comprising
- (a2) said one or more bile acid derivatives or salts thereof;
- (b2) a triglyceride hydrolysing reagent;
- (c2) glycerol dehydrogenase;
- (d2) a coenzyme;
- and
- (e2) a redox agent capable of being oxidised or reduced to form a product;
- and electrochemically detecting the amount of product formed.
A method comprising:
- providing a liquid sample containing an amount of triglycerides, said triglycerides being bound to lipoproteins;
- freeing the triglycerides from the lipoproteins within the liquid sample by contacting the triglycerides with a surfactant comprising bile acid derivatives or salts thereof configured to break the lipoproteins from the triglycerides;
- breaking down the freed triglycerides produced within the liquid sample into a proportionate amount of glycerol by reacting the freed triglycerides with a triglyceride hydrolyzing reagent;
- and
- electrochemically determining the amount of glycerol in the liquid sample by:
- reacting the glycerol with a suitable enzyme or enzyme-coenzyme reagent in the presence of a redox mediator;
- applying an electrical potential to the liquid sample;
- measuring the resulting electrochemical response;
- and
- correlating the measured response to the amount of glycerol;
- wherein the method provides an indication of the total triglyceride content of the liquid sample.
Dependencies are inferred from the claim text.