Lateral flow assays
AssigneeRapid Pathogen Screening, Inc.
InventorsUma Mahesh Babu, Robert P. Sambursky, Robert W. VanDine
Life of this patent
Estimating the term…
Abstract
Assays and methods including mobile tagged single stranded nucleic acid reagents pre-loaded on an analysis device, which are preferably tagged, but not labeled and are complementary to a strand (preferably the anti-sense strand in double stranded DNA targets) of the target nucleic acid. The assay also includes a running buffer that includes a dye or other detectable label that nonspecifically binds only to double stranded nucleic acids. In addition, the analysis device includes a detection zone including one or more test zones that have an immobilized tag that binds to the tag on the mobile nucleic acid reagent.
Claims27
A test strip for detection of at least one target nucleic acid in a sample comprising:
- at least one detection zone comprising at least one test zone;
- a sample application zone for applying the sample to the test strip;
- at least one mobile tagged nucleic acid reagent comprising a plurality of nucleic acid sequences complementary to a first portion of a sequence of the target nucleic acid and at least one tag portion comprising a first tag, wherein the mobile tagged nucleic acid reagent does not include a detectable label and wherein the mobile tagged nucleic acid reagent is loaded in a reagent zone;
- and
- at least one second immobilized tag that binds to the first tag, wherein the immobilized tag is immobilized in a test zone on the test strip;
- and
- wherein the sample, the mobile tagged nucleic acid reagent, and the immobilized tag are all loaded on the test strip at locations such that the sample encounters the mobile tagged nucleic acid reagent and the immobilized tag while running an assay to detect the target nucleic acid in the sample.
A method of detecting at least one target nucleic acid in a sample using a point-of-care assay, the method comprising the steps of:
- a) exposing the sample to at least one mobile tagged nucleic acid reagent comprising a plurality of nucleic acids complementary to a portion of a sequence of the target nucleic acid and a first tag but not a detectable label;
- and
- b) exposing the sample to a detectable label, wherein the label nonspecifically binds to double stranded nucleic acids;
- wherein, when the target nucleic acid is present in the sample, the mobile tagged nucleic acid reagent binds to the target nucleic acid sequence and the first tag of the mobile tagged nucleic acid reagent binds to a second immobilized tag in a test zone of the assay at a completion of the assay.
A test kit for detection of at least one target nucleic acid in a sample comprising:
- a test strip comprising:
- at least one detection zone comprising at least one test zone;
- a sample application zone for applying the sample to the test strip;
- at least one mobile tagged nucleic acid reagent comprising a plurality of nucleic acids complementary to a first portion of a sequence of the target nucleic acid and at least one tag portion but not including a label, wherein the mobile tagged nucleic acid reagent is loaded in a reagent zone;
- at least one lysis agent loaded in a lysis zone wherein the lysis agent processes the sample such that the target nucleic acid can bind to the mobile tagged nucleic acid reagent when the target nucleic acid encounters the mobile tagged nucleic acid reagent;
- and
- at least one immobilized tag that binds to the tag portion of the mobile tagged nucleic acid reagent, wherein the immobilized tag is immobilized in a test zone of an assay;
- and
- a running buffer comprising at least one detectable label that nonspecifically binds to double stranded nucleic acids.
Dependencies are inferred from the claim text.