Methods for Evaluating a Disease Condition by Nucleic Acid Detection and Fractionation
AssigneeThe Chinese University of Hong Kong
InventorsYuk Ming Dennis Lo, Kai On Ng, Bo Yin Tsui, Wai Kwun Rossa Chiu, Yuen Shan Lisa Chan, Timothy Hudson Rainer, Yuk Lan Lam
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Abstract
This invention relates to the discovery that both non-particle and particle associated nucleic acids are present in blood plasma and serum and can be used to evaluate disease conditions.
Claims22
A method of evaluating a disease condition in a patient suspected of suffering or known to suffer from the disease condition, said method comprising:
- (i) obtaining a blood sample from the patient suspected of suffering or known to suffer from a disease condition,
- (ii) isolating plasma or serum from the blood sample,
- (iii) separating the plasma or serum into two or more fractions containing different relative concentrations of particle-associated and non-particle associated nucleic acid, and
- (iv) evaluating the disease condition by determining the amount or concentration or characteristic of nucleic acid in one or more fractions and comparing the amount or concentration or characteristic of nucleic acid in one or more fractions to a control.
A method of evaluating the disease condition of a patient suspected of having or known to have or at risk of having hepatocellular cancer or a nasopharyngeal carcinoma, the method comprising:
- (i) obtaining a sample of plasma or serum from the patient suspected of having or known to have hepatocellular cancer or a nasopharyngeal carcinoma, and
- (ii) detecting the quantity of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) nucleic acid in the sample.
A method of evaluating the disease condition of a patient suspected of having suffered from a trauma or known to have suffered from a trauma, the method comprising:
- (i) obtaining a sample of plasma or serum from the patient suspected of having suffered from a trauma or known to have had suffered from a trauma, and
- (ii) detecting the quantity or concentration of mitochondrial nucleic acid in the sample.
Dependencies are inferred from the claim text.