Proliferation Signatures and Prognosis for Gastrointestinal Cancer

A method of predicting the likelihood of long-term survival of a gastrointestinal cancer patient without the recurrence of gastrointestinal cancer, comprising determining the expression level of one or more prognostic RNA transcripts or their expression products in a gastrointestinal sample obtained from the patient, normalized against the expression level of all RNA transcripts or their products in the gastrointestinal cancer tissue sample, or of a reference set of RNA transcripts or their expression products;

• wherein the prognostic RNA transcript is the transcript of one or more genes selected from table A, Table B, Table C or Table D;
• and
• establishing likelihood of long-term survival without gastrointestinal cancer recurrence.

An array comprising polynucleotides hybridizing to two or more genes selected from table A, Table B, Table C or Table D.

The array ofclaim 13 wherein the solid surface is glass.

A method of preparing a personalized genomics profile for a cancer patient, comprising the steps of (a) subjecting RNA extracted from a gastrointestinal tissue obtained from the patient to gene expression analysis;

• (b) determining the expression level of one or more genes selected from the gastrointestinal cancer gene set listed in any one of Table A, Table 13, Table C or Table D, wherein the expression level is normalized against a control gene or genes and optionally is compared to the amount found in a gastrointestinal cancer reference tissue set;
• and (c) creating a report summarizing the data obtained by the gene expression analysis.

A prognostic method comprising:

• (a) subjecting a sample comprising gastrointestinal cancer cells obtained from a patient to quantitative analysis of the levels of RNA transcripts of at least one gene selected from any one of Table A, Table B, Table C or table D, or its product, and (b) identifying the patient as likely to have an increased likelihood of long-term survival without gastrointestinal cancer recurrence if normalized expression levels of the gene or genes, or their products, are elevated above a defined expression threshold.