Loading patent…
AssigneeMurdoch Childrens Research Institute
InventorsDamien Luis Bruno, Howard Robert Slater
Estimating the term…
The present invention relates generally to a method for identifying or distinguishing one type of cell from other cells within a population of cells. The present invention further provides the detection, monitoring and isolation of sub-populations cell types within a population of cells including a biological entity comprising such cell types. Kits, diagnostic agents and panels of nucleic acid probes for identifying and distinguishing cell types also form part of the present invention. The detection of particular cell types is based upon the use of a labelled probe that hybridizes to chromosomal DNA at the flanking sequences of a deletion. Alternatively two probes with distinguishable reporter molecules are used wherein only one probe is capable of hybridizing to chromosomal DNA in one cell type whereas both are capable of hybridizing to chromosomal DNA in another cell type. The methods are useful in the identification of cells with copy number variations, chromosomal deletions, additions or aberrations.
A method for identifying a cell type in a sample comprising a mixture of cell types from a subject, said method selected from the list consisting of:
(canceled)
(canceled)
16-38. (canceled)
A method of treatment or monitoring a treatment, the method comprising collecting a sample comprising cells to be transplanted to a subject and contacting the cells with a nucleic acid probe labeled with a reporter molecule capable of giving an identifiable signal which probe is capable of hybridizing to chromosomal DNA at the flanking sequences of a deletion in one cell type, which deletion absent in chromosomal DNA in another cell type, wherein the presence or absence of a signal in a cell is indicative of a particular cell type expanding the desired cells ex vivo and then transplanting the cells into the same subject or a different subject and then using the same method to monitor distribution of said cells.
A method of treatment or monitoring a treatment, the method comprising collecting a sample comprising cells to be transplanted to a subject, contacting the cells with two nucleic acid probes each labeled with distinguishable reporter molecules each capable of giving an identifiable signal wherein one nucleic acid probe is capable of hybridizing to chromosomal DNA from one cell type whereas both nucleic acid probes are capable of hybridizing to a portion of chromosomal DNA present in another cell type, wherein the presence or absence of a single signal or a combination of signals in a cell is indicative of the cell types expanding the desired cells ex vivo and then transplanting the cells into the same subject or a different subject and then using the same method to monitor distribution of said cells.
(canceled)
An assay to detect a potential genetic abnormality in fetal cells said method selected from the list consisting of:
Dependencies are inferred from the claim text.