Methods of characterizing small nucleic acid molecules
AssigneeOpgen, Inc.
InventorsRonald W. Lennox, Colin W. Dykes
Life of this patent
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Abstract
The invention generally relates to methods of characterizing small nucleic acid molecules, such as plasmids or nucleic acid molecules obtained from a virus. In certain embodiments, the invention provides a method of characterizing a small circular nucleic acid molecule, the method including performing rolling circle amplification on a small circular nucleic acid molecule, thereby producing a concatamer, and generating an optical map of the concatamer, thereby characterizing the small circular nucleic acid molecule.
Claims21
A method of characterizing a small circular nucleic acid molecule, the method comprising:
- performing rolling circle amplification on a small circular nucleic acid molecule, thereby producing a concatamer;
- and
- generating an optical map of the concatamer, thereby characterizing the small circular nucleic acid molecule.
A method of characterizing a small linear nucleic acid molecule, the method comprising:
- circularizing a small linear nucleic acid molecule to produce a circular nucleic acid molecule;
- performing rolling circle amplification on the circular nucleic acid molecule, thereby producing a concatamer;
- and
- generating an optical map of the concatamer, thereby characterizing the small linear nucleic acid molecule.
A method of characterizing a small circular nucleic acid molecule, the method comprising:
- performing rolling circle amplification on a small circular nucleic acid molecule, thereby producing a concatamer;
- depositing the concatamer onto a surface such that the concatamer is elongated and fixed on the surface so that the concatamer remains accessible for enzymatic reactions;
- digesting the concatamer enzymatically to produce one or more restriction digests;
- imaging the restriction digests;
- and
- constructing an optical map from the restriction digests.
Dependencies are inferred from the claim text.