Bacteriophage/Quantum-Dot (Phage-QD) Nanocomplex to Detect Biological Targets in Clinical and Environmental Isolates
OwnerJeeseong Hwang, Rotem Edgar +4inventors
InventorsJeeseong Hwang, Rotem Edgar, Michael Mckinstry, Gary Giulian, Carl Merril, Sankar Adhya
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Abstract
The invention is related to a non-biotinylated bacteriophage that comprises a nucleic acid sequence encoding a biotinylation domain, a complex that comprises a biotinylated bacteriophage and a biotin-specific ligand conjugated bioconjugate, and a method of detecting a bacterial cell in a sample comprising contacting the sample with a non-biotinylated bacteriophage that comprises a nucleic acid sequence encoding a biotinylation domain, wherein the bacteriophage is specific to the bacterial cell.
Claims20
A non-biotinylated bacteriophage that comprises a nucleic acid sequence encoding a biotinylation domain.
A complex that comprises:
- a) a biotinylated bacteriophage, and
- b) a biotin-specific ligand conjugated bioconjugate.
A method of detecting a bacterial cell in a sample comprising:
- contacting the sample with a non-biotinylated bacteriophage that comprises a nucleic acid sequence encoding a biotinylation domain, wherein the bacteriophage is specific to the bacterial cell.
A bacteriophage engineered to have the major head, capsid protein assembly of the phage express a first attachment site.
A complex that comprises:
- a) a bacteriophage engineered to have the major head, capsid protein assembly of the phage express a first attachment site, and
- b) a bioconjugate functionalized with a second attachment site capable of recognizing/binding the first attachment, site expressed on the engineered phage.
Dependencies are inferred from the claim text.