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US 2011/0086181 A1Published application
Dna extraction using a photo-polymerized monolith in a capillary
AssigneeUniversity of Virginia Patent Foundation
InventorsJian Wen, Jerome P. Ferrance, James P. Landers
Life of this patent
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Abstract
The present invention relates to the fabrication of a grafted, UV photo-polymerized silica-based monolithic column and the use of such column for the extraction of DNA. In one embodiment, a method is provided for fabricating a silica-based monolithic column, wherein a vessel is filled with a polymerization mixture that is formed into monolithic solid phase for DNA extraction through in situ photo-polymerization.
Claims21
1-30. (canceled)
A method for preparing a monolithic column comprising the steps of:
- (i) providing a vessel;
- (ii) conditioning the interior surface of said vessel by contacting it with a silica-based flushing solution;
- (iii) preparing a silica-based monomer solution through hydrolysis of a silica monomer;
- (iv) forming a polymerizable mixture by admixing said silica-based monomer solution, an initiator material, and a porogenic solvent;
- (v) introducing said polymerizable mixture into the vessel;
- (vi) initiating the in situ polymerization of said polymerizable mixture by exposing selected portions of the mixture to UV light, thereby forming the UV photo-polymerized silica-based monolithic column within portions of the vessel;
- (vii) flushing a silica-based monomer reagent through said UV photo-polymerized silica-based monolithic column, thereby forming the grafted porous UV photo-polymerized silica-based monolithic column within portions of the vessel
4 more elements
Dependencies are inferred from the claim text.