Method for producing biomolecule diagnostic devices

A method of making a biosensor, comprising the steps of:

• applying a photo-reactive crosslinking agent to a surface of a substrate member;
• forming one of a receptive material layer and a blocking material layer over the crosslinking agent;
• placing a mask over the substrate member, the mask having a configuration so as to shield at least one underlying area of the substrate member while exposing at least one adjacent area, and irradiating the substrate member and mask combination with an energy source sufficient to activate the crosslinking agent in the areas exposed by the mask, the activated crosslinking agent crosslinking with the respective receptive material or blocking material in the exposed areas;
• cleaning the unreacted receptive material or blocking material from the shielded areas of the substrate member after removal of the mask;
• forming a layer of the respective other of the blocking material and receptive material over the surface of the substrate member, and irradiating the substrate member with the energy source so as to activate the remaining crosslinking agent in the previously shielded areas, the activated crosslinking agent crosslinking with the respective blocking material or receptive material;
• and
• wherein a resulting pattern of active receptive material areas and blocking material areas are defined according to the pattern of shielded and exposed areas of the mask.