Lipolytic enzyme: uses thereof in the food industry
AssigneeDupont Nutrition Biosciences Aps
InventorsAndrei Miasnikov, Jørn Borch Søe, Jørn Dalgaard Mikkelsen, Mira Povelainen, Virve Pitkanen
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Abstract
The invention encompasses the use of a lipolytic enzyme obtainable from one of the following genera: Streptomyces, Corynebacterium and Thermobifida in various methods and uses, wherein the lipolytic enzyme hydrolyzes a glycolipid or a phospholipid or transfers an acyl group from a glycolipid or phospholipids to an acyl acceptor. The present invention also relates to a lipolytic enzyme that hydrolyzes at least a galactolipid or transfers an acyl group from a galactolipid to one or more acyl acceptor substrates, wherein the enzyme is obtainable from Corynebacterium species.
Claims26
1-19. (canceled)
A method of preparing a lysoglycolipid comprising treating a substrate comprising a glycolipid with at least one lipolytic enzyme to produce said lysoglycolipid, wherein said lipolytic enzyme has glycolipase activity and wherein said lipolytic enzyme is obtainable from the genus Corynebacterium.
A method of preparing a lysophospholipid comprising treating a substrate comprising a phospholipid with at least one lipolytic enzyme to produce said lysophospholipid, wherein said lipolytic enzyme has phospholipase activity and wherein said lipolytic enzyme is obtainable from the genus Corynebacterium.
A method of enzymatic degumming of vegetable or edible oil, comprising treating said edible or vegetable oil with a lipolytic enzyme obtainable from the genus Corynebacterium capable of hydrolysing a major part of the polar lipids.
A method of bioconversion of polar lipids to make high value products comprising treating said polar lipids with a lipolytic enzyme obtainable from the genus Corynebacterium to produce said high value products, wherein said lipolytic enzyme is capable of hydrolysing said polar lipids.
A method of preparing a foodstuff comprising admixing at least one lipolytic enzyme with one or more ingredients of a foodstuff wherein said lipolytic enzyme is capable of hydrolysing a glycolipid and/or a phospholipid present in or as at least one of said ingredients and wherein said lipolytic enzyme is obtainable from the genus Corynebacterium.
(canceled)
(canceled)
35-37. (canceled)
A method of hydrolyzing fatty acyl groups in a phospholipid comprising contacting the phospholipid with a lipolytic enzyme obtainable from Corynebacterium, wherein the lipolytic enzyme hydrolizes the fatty acyl groups in a phospholipid.
39-44. (canceled)
The method according to claims 43 wherein said lipolytic enzyme comprises an amino acid sequence shown as SEQ ID No 8 or an amino acid sequence having at least 70% identity therewith.
(canceled)
Dependencies are inferred from the claim text.