Vectors Containing the Max Gene
AssigneeUniversidade Federal Do Rio De Janeiro
InventorsRafael Linden, Hilda Petrs Silva, Luciana Barreto Chiarini
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Abstract
The present invention refers to the construction of cloning vectors containing the max gene. Especially, the present invention refers to the introduction of cloning vectors containing the max gene in cells using transport vectors. In addition, the presence of cloning vectors containing the max gene in cells allows the differential expression of the max gene in the same cells. In addition, the present invention refers to a method of gene therapy in which the differential expression of the max gene has cytoprotective activity, especially neuroprotective activity, and is capable of application to medical and veterinary therapeutics of neurodegenerative conditions.
Claims28
A biological vector comprising a max gene and containing:
- a) a cloning vector comprising:
- a.1) a nucleotide sequence capable of expressing the max gene or its fragments;
- and
- a.2) an adequate promoter;
- and
- b) one or more transport vectors chosen from a group consisting of viral transport vectors, non-viral transport vectors, and mixtures of the foregoing.
A method for the production of biological vectors containing a max gene, comprising the stages of:
- a) providing a cloning vector comprising:
- a.1) a nucleotide sequence capable of expressing the max gene or its fragments;
- and
- a.2) an adequate promoter;
- b) providing a biological vector chosen among viral transport vectors, non-viral transport vectors and mixtures thereof;
- and
- c) contacting said biological vector with said cloning vector.
A method for the expression of the max gene in cells, comprising the stage of contacting the biological vectors with an adequate cell, where the biological vector comprises:
- a) a cloning vector containing:
- a.1) a nucleotide sequence capable of expressing the max gene or its fragments;
- and
- a.2) an adequate promoter;
- and
- b) one or more transport vectors selected from a group consisting of viral transport vectors, non-viral transport vectors and mixtures of both.
A method of gene therapy comprising a stage of introducing a biological vector containing a max gene into at least one target cell of a higher animal;
- in order to modulate the cellular activity of the target cell.
Dependencies are inferred from the claim text.