Methods for Producing Hair Microfollicles and De Novo Papillae and Their Use for In Vitro Tests and In Vivo Implantations
AssigneeTechnische UniversitäT Berlin
InventorsGerd Lindner, Roland Lauster
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Abstract
The present invention relates to a method for producing hair microfollicles comprising the steps of: a) providing de novopapillae, b) providing other cell populations selected from the group of fibroblasts, keratinocytes and melanocytes, and co-culturing the de novopapillae with at least one other cell population in non-adherent culture vessels. The present invention relates also to methods of producing de novo papillae usable in said method for producing hair microfollicles.
Claims28
1-20. (canceled)
A method for producing mammalian hair microfollicle comprising the steps of:
- (a) providing at least one de novo papilla,
- (b) providing at least one other cell population selected from the group of fibroblasts, keratinocytes and/or melanocytes, and
- (c) co-culturing the de novo papilla with the at least one other cell population under non-adherent culture conditions.
The method according to claim 21, wherein de novo papillae are produced according to the method of claim 27.
A method for producing de novo papillae comprising the steps of:
- (a) providing at least one dermal papilla (DP) from at least one mammal hair follicle,
- (b) isolating dermal hair papilla fibroblasts (DPFs) from the DP by mechanically fixing said DP at the surface of a cell culture vessel, whereby the basal lamina is perforated to allow said DPFs migrating out,
- (c) expanding the isolated DPFs in monolayer culture without collagen coating, wherein said DPFs are passaged at least once,
- (d) condensing the expanded DPFs into cell aggregates that exhibit the size and shape of the physiological DP, wherein said DPFs are differentiated in non-adhesive culture vessels in a cell concentration per vessel surface of 1,000 to 100,000 DPFs/cm2, and
- (e) coating the de novo papillae with extracellular matrix proteins, preferably collagen IV, fibronectin and/or laminin.
Dependencies are inferred from the claim text.