Compositions and Methods for Tissue Engineering, Tissue Regeneration and Wound Healing
AssigneesMedical University of South Carolina, University of South Carolina
InventorsRobert G. Gourdie, Jay D. Potts
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Abstract
In accordance with certain embodiments of the present disclosure, a kit is described. The kit includes primed living cells joined to and at least partially within a three-dimensional hydrogel structure and an isolated polypeptide having the carboxy-terminal amino acid sequence of an alpha Connexin, or a conservative variant thereof, wherein the polypeptide does not include the full length alpha Connexin protein.
Claims20
A kit comprising:
- primed living cells joined to and at least partially within a three-dimensional hydrogel structure;
- and
- an isolated polypeptide comprising the carboxy-terminal amino acid sequence of an alpha Connexin, or a conservative variant thereof, wherein the polypeptide does not comprise the full length alpha Connexin protein.
A method for forming a hydrogel structure in which living cells are joined thereto comprising:
- placing living cells on a three-dimensional hydrogel structure;
- and
- permitting epithelialization of the cells in which the cells attach to the surface of the hydrogel structure, undergo epithelial mesenchymal transition, and penetrate the surface of the hydrogel structure.
A method of promoting healing following tissue injury in a subject, comprising:
- administering to the subject's injury primed living cells which are joined to and at least partially within a three-dimensional hydrogel structure and an isolated polypeptide comprising the carboxy-terminal amino acid sequence of an alpha Connexin, or a conservative variant thereof, wherein the polypeptide does not comprise the full length alpha Connexin protein.
A method of epithelial mesenchymal transition priming of cells comprising:
- placing living cells on a culture;
- and
- permitting epithelialization of the cells in which the cells attach to the surface of the culture, undergo epithelial mesenchymal transition, and penetrate the surface of the culture.
Dependencies are inferred from the claim text.