Yeast strain for the production of proteins with terminal alpha-1,3-linked galactose
OwnerNatarajan Sethuraman, Robert C. Davidson +2inventors
InventorsNatarajan Sethuraman, Robert C. Davidson, Terrance A. Stadheim, Stefan Wildt
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Abstract
Lower eukaryotic host cells have been engineered to produce glycoprotein having at least one terminal α-galactosyl epitope. The glycoproteins are useful for the production of highly antigenic glycoprotein compositions with advantages for the production of vaccines.
Claims17
A yeast or filamentous fungus host cell that can produce recombinant glycoproteins having N-glycans that have at least one terminal α-galactosyl epitope.
A method for producing a host cell capable of producing N-glycans that have terminal α-galactosyl residues, comprising:
- (a) providing a recombinant yeast or filamentous fungus host cell capable of producing N-glycans that have at least one terminal α-galactose on the non-reducing end of the N-glycan;
- and
- (b) introducing a nucleic acid encoding an fusion protein comprising the catalytic domain of an α-galactosyltransferase linked to a targeting peptide that targets the fusion protein to the ER or Golgi of the host cell into the host cell to provide the host cell producing N-glycans that have terminal α-galactosyl residues.
(canceled)
A recombinant glycoprotein produced by a yeast or filamentous fungal host cell, wherein the N-glycans of said glycoprotein comprises a predominant N-glycan comprising a terminal α-galactosyl epitope.
Dependencies are inferred from the claim text.