Node polypeptides for nanostructure assembly
AssigneeImiplex LLC
InventorsF. Raymond Salemme, Patricia C. Weber, Mark A. Rould
Life of this patent
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Abstract
Engineered proteins are used in the assembly of two-dimensional and three-dimensional nanostructure assemblies, based on systematic design and production of protein node structures that can be interconnected, for example, with streptavidin or streptavidin-incorporating struts to produce structures with defined dimensions and geometry. Nanostructure assemblies having utility as functional devices or as resists for the patterning of substrates have architectures including polygons, polyhedra, two-dimensional lattices, and three-dimensional lattices.
Claims74
A nanostructure node, comprising
- a multimeric protein having at least one polypeptide chain and a known three-dimensional structure with Cn, Dn, or higher symmetry,
- wherein the multimeric protein has stable tertiary structure at a temperature of 70° C. or greater and a modified amino acid sequence comprising a specific binding site positioned with predefined stoichiometry and orientation for attachment of a nanostructure strut,
- wherein the specific binding site comprises two reactive amino acid residues, each covalently attached to a moiety independently selected from the group consisting of a biotin group, a biotin derivative group, iminobiotin, an adenosine triphosphate (ATP) group, an adenosine triphosphate (ATP) derivative group, a nucleobase group, a nucleobase derivative group, a nucleoside group, a nucleoside derivative group, a nucleotide group, and a nucleotide derivative group.
A nanostructure node chain:streptavidin complex, comprising:
- a polypeptide chain of a nanostructure node;
- and
- a streptavidin or streptavidin derivative tetramer having biotin binding sites,
- wherein the nanostructure node comprises a multimeric protein having a known three-dimensional structure, with Cn, Dn, or higher symmetry,
- wherein the multimeric protein has stable tertiary structure at a temperature of 70° C. or greater and a modified amino acid sequence,
- wherein the polypeptide chain comprises two cysteine residues, each functionalized with a biotin or biotin derivative group, and
- wherein the biotin or biotin derivative groups of the two cysteine residues are bound to two biotin binding sites of the streptavidin or streptavidin derivative tetramer.
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A nanostructure node, comprising:
- a modified amino acid sequence that has a stable tertiary structure at a temperature of 70° C. or greater, a known 3-dimensional structure that essentially has Cn, Dn, or higher symmetry, and a specific binding site for the attachment of a nanostructure strut with predefined stoichiometry and orientation,
- wherein the specific binding site comprises at least two specific reactive amino acid residues.
Dependencies are inferred from the claim text.