Primer and probe for detection of mycobacterium intracellulare, and method for detection of mycobacterium intracellulare using the primer or the probe
AssigneeFujifilm Wako Pure Chemical Corporation
InventorTomokazu Ishikawa
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Abstract
The present invention relates to an oligonucleotide, comprising a part or the entire of the nucleotide sequence shown in any of SEQ ID NO: 1 to SEQ ID NO: 15, or a part or the entire of the sequence complementary to the nucleotide sequence shown in any of SEQ ID NO: 1 to SEQ ID NO: 15, wherein the oligonucleotide is capable of hybridizing with the nucleotide sequence of Mycobacterium intracellulare (M. intracellulare) gene; a primer or a probe for the detection of M. intracellulare which comprises said oligonucleoride; and a method for detection of M. intracellulare using said primer and/or the probe.
Claims19
An oligonucleotide comprising a part or the entire of the nucleotide sequence shown in any of SEQ ID NO:
- 1 to SEQ ID NO:
- 15 (wherein the characters of A, C, G and T represent adenine, cytosine, guanine and thymine, respectively;
- and, T at arbitrary position may be replaced by uracil (U);
- and hereinafter, the same as above), or a part or the entire of the sequence complementary to the nucleotide sequence shown in any of SEQ ID NO:
- 1 to SEQ ID NO:
- 15,
- wherein the oligonucleotide is capable of hybridizing with a nucleotide sequence of Mycobacterium intracellulare gene.
A primer for detecting Mycobacterium intracellulare, comprising;
- an oligonucleotide comprising a part or the entire of the nucleotide sequence shown in any of SEQ ID NO:
- 1 to SEQ ID NO:
- 15, or a part or the entire of the sequence complementary to the nucleotide sequence shown in any of SEQ ID NO:
- 1 to SEQ ID NO:
- 15,
- wherein the oligonucleotide is capable of hybridizing with a nucleotide sequence of Mycobacterium intracellulare gene.
A probe for detecting Mycobacterium intracellulare, comprising;
- an oligonucleotide comprising a part or the entire of the nucleotide sequence shown in any of SEQ ID NO:
- 1 to SEQ ID NO:
- 15, or a part or the entire of the sequence complementary to the nucleotide sequence shown in any of SEQ ID NO:
- 1 to SEQ ID NO:
- 15,
- wherein the oligonucleotide is capable of hybridizing with the nucleotide sequence of Mycobacterium intracellulare gene.
A method for detecting Mycobacterium intracellulare, comprising:
- using an oligonucleotide comprising a part or the entire of the nucleotide sequence shown in any of SEQ ID NO:
- 1 to SEQ ID NO:
- 15, or a part or the entire of the sequence complementary to the nucleotide sequence shown in any of SEQ ID NO:
- 1 to SEQ ID NO:
- 15, wherein the oligonucleotide is capable of hybridizing with a nucleotide sequence of Mycobacterium intracellulare gene as a primer or/and a probe.
A reagent kit for detecting Mycobacterium intracellulare, comprising:
- an oligonucleotide comprising a part or the entire of the nucleotide sequence shown in any of SEQ ID NO:
- 1 to SEQ ID NO:
- 15, or a part or the entire of the sequence complementary to the nucleotide sequence shown in any of SEQ ID NO:
- 1 to SEQ ID NO:
- 15, wherein the oligonucleotide is capable of hybridizing with the nucleotide sequence of Mycobacterium intracellulare gene, as a primer or/and a probe.
Dependencies are inferred from the claim text.