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US 2011/0076760 A1Published application
Viral expression plasmids for production of proteins, antibodies, enzymes, virus-like particles and for use in cell-based assays
AssigneeIcosagen S.A.
InventorsToomas Silla, Ingrid Tagen, Anne Kalling, Radi Tegova, Mart Ustav, Tiiu Mandel, Urve Toots, Andres Tover, Aare Abroi, Ene Ustav, Jelizaveta Geimanen, Kadri Janikson
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Abstract
This disclosure shows that the EBV FR-element comprised of EBNA1 multimeric binding sites can provide the stable maintenance replication function to the mouse polyomavirus (PyV) core origin plasmids in the presence of BPV-1 E2 protein and PyV large T-antigen (LT).
Claims21
An expression system to provide extended episomal replication of hybrid plasmid in eukaryotic cell lines, said system comprising:
- a) a vector comprising a polyoma virus core origin, an FR element of EBV, a selection marker, and a gene of interest operably linked within an expression cassette expressing the gene in a eukaryotic cell;
- and
- b) a compatible mammalian cell line, where the vector is expressed, said cell line constitutively expressing EBV EBNA1 protein and PyV LT protein either in presence or in absence of selective pressure.
An expression plasmid for use in a mammalian cell expression system, said vector comprising:
- a) a polyoma virus core origin,
- b) an FR element of Epstein Barr Virus (EBV);
- c) a selection marker;
- and
- d) an expression cassette for expression a gene of interest in a eukaryotic cell.
2 more elements
Dependencies are inferred from the claim text.