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US 2011/0053215 A1Published application
Endoglycosidases that Cleave O-linked Glycans
AssigneeNew England Biolabs, Inc.
InventorsDimitris Koutsioulis, Ellen Guthrie
Life of this patent
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Abstract
Methods and compositions have been described that relate to a newly identified polypeptide family wherein each member has O-glycosidase activity and specified sequence characteristics. This family of enzymes can be used for example for cleaving O-linked glycans and for synthesis of neoglycopeptides or neoglycoproteins.
Claims8
A polypeptide family in which each polypeptide member is characterized by:
- (i) O-glycosidase activity;
- (ii) an amino acid sequence homology with EngBF measured by an expectation value of less than e−10 in a BLAST search;
- (iii) an amino acid sequence comprising SEQ ID NO:1.;
- and
- (iv) an FDY amino acid sequence in a central conserved domain wherein the number of amino acids between the FDY and a C-terminal end of the polypeptide member is no more than 200 amino acids.
2 more elements
Dependencies are inferred from the claim text.