Zona Pellucida Binding Peptides, Expression Vectors, Compositions, and Methods for Species Specific Immunocontraception of Animals
AssigneeAuburn University
InventorsTatiana I. Samoylova, Henry J. Baker, Nancy Cox, Stephen Ditchkoff, Kent Van Kampen
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Abstract
Disclosed are methods, compositions, zona pellucida binding peptides and polypeptides, and expression vectors for use in species-specific immunocontraception of animals. The disclosed compositions may include immunogenic compositions or vaccines.
Claims27
A method for identifying a peptide that binds specifically to zona pellucida of oocytes of a target species of animal, the method comprising
- (a) isolating oocytes of the target species of animal and isolating oocytes of one or more non-target species of animals;
- (b) contacting the isolated oocytes with a phage library;
- (c) selecting phage from the library that bind specifically to the oocytes of the target species of animal, thereby identifying peptides that bind to the zona pellucida of oocytes of the target species of animal.
An isolated polypeptide comprising an amino acid sequence selected from a group consisting of SEQ ID NO:2 (DANRLPHPANIN), SEQ ID NO:18 (TLGWTANEAPRR), SEQ ID NO:
- 19 (LLADTTHHRPWT), SEQ ID NO:20 (SQSPAMYSQTRP), SEQ ID NO:21 (AVTQHLKFKGFN), and SEQ ID NO:22 (ANFNMTHHQGHK).
An isolated polypeptide consisting of an amino acid sequence selected from SEQ ID NO:2 (DANRLPHPANIN), SEQ ID NO:18 (TLGWTANEAPRR), SEQ ID NO:19 (LLADTTHHRPWT), SEQ ID NO:20 (SQSPAMYSQTRP), SEQ ID NO:21 (AVTQHLKFKGFN), and SEQ ID NO:22 (ANFNMTHHQGHK).
An immunogenic composition comprising:
- (a) a polypeptide comprising an amino acid sequence selected from a group consisting of SEQ ID NO:2 (DANRLPHPANIN), SEQ ID NO:18 (TLGWTANEAPRR), SEQ ID NO:19 (LLADTTHHRPWT), SEQ ID NO:20 (SQSPAMYSQTRP), SEQ ID NO:21 (AVTQHLKFKGFN), and SEQ ID NO:22 (ANFNMTHHQGHK);
- and
- (b) a suitable excipient, carrier, or diluent.
Dependencies are inferred from the claim text.