US 2011/0035847 A1Published application

Yield traits for maize

AssigneeSyngenta Participations AG

InventorsVenkata Krishna Kishore, Libardo Andres Gutierrez Rojas, Zhigang Guo, Joseph Byrum

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Abstract

Methods for introgressing an allele of interest of a locus associated with a yield trait into Zea mays germplasm are provided. In some embodiments, the methods include providing a Zea mays plant that contains an allele of interest of a locus associated with a yield trait, wherein the locus associated with the yield trait is identifiable by PCR amplification of a Zea mays nucleic acid with a pair of oligonucleotides primers as disclosed herein, and introgressing the allele of interest into Zea mays germplasm that lacks the allele. Also provided are methods for identifying Zea mays plants that contain at least one allele associated with improved yield, improved maize plants, elite Zea mays plants, biomass produced from improved Zea mays plants, isolated and purified genetic markers, and compositions that include an amplification primer pair capable of amplifying a Zea mays nucleic acid to generate a Zea mays marker amplicon.

Claims18

Dependent claims

A method for introgressing an allele of interest of a locus associated with a yield trait into Zea mays germplasm, the method comprising:

(a) selecting a Zea mays plant that comprises an allele of interest of a locus associated with a yield trait, wherein the locus associated with the yield trait is identifiable by PCR amplification of a Zea mays nucleic acid with a pair of oligonucleotides primers selected from among:
(i) primer pair 1 represented by a primer comprising SEQ ID NO:
2 and a primer comprising SEQ ID NO:
3;
(ii) primer pair 2 represented by a primer comprising SEQ ID NO:
5 and a primer comprising SEQ ID NO:
6;
(iii) primer pair 3 represented by a primer comprising SEQ ID NO:
8 and a primer comprising SEQ ID NO:
9;
(iv) primer pair 4 represented by a primer comprising SEQ ID NO:
11 and a primer comprising SEQ ID NO:
12;
(v) primer pair 5 represented by a primer comprising SEQ ID NO:
14 and a primer comprising SEQ ID NO:
15;
(vi) primer pair 6 represented by a primer comprising SEQ ID NO:
17 and a primer comprising SEQ ID NO:
18;
(vii) primer pair 7 represented by a primer comprising SEQ ID NO:
20 and a primer comprising SEQ ID NO:
21;
(viii) primer pair 8 represented by a primer comprising SEQ ID NO:
23 and a primer comprising SEQ ID NO:
24;
(ix) primer pair 9 represented by a primer comprising SEQ ID NO:
26 and a primer comprising SEQ ID NO:
27;
(x) primer pair 10 represented by a primer comprising SEQ ID NO:
29 and a primer comprising SEQ ID NO:
30;
(xi) primer pair 11 represented by a primer comprising SEQ ID NO:
32 and a primer comprising SEQ ID NO:
33;
(xii) primer pair 12 represented by a primer comprising SEQ ID NO:
35 and a primer comprising SEQ ID NO:
36;
(xiii) primer pair 13 represented by a primer comprising SEQ ID NO:
38 and a primer comprising SEQ ID NO:
39;
(xiv) primer pair 14 represented by a primer comprising SEQ ID NO:
41 and a primer comprising SEQ ID NO:
42;
(xv) primer pair 15 represented by a primer comprising SEQ ID NO:
44 and a primer comprising SEQ ID NO:
45;
(xvi) primer pair 16 represented by a primer comprising SEQ ID NO:
47 and a primer comprising SEQ ID NO:
48;
(xvii) primer pair 17 represented by a primer comprising SEQ ID NO:
50 and a primer comprising SEQ ID NO:
51;
(xviii) primer pair 18 represented by a primer comprising SEQ ID NO:
53 and a primer comprising SEQ ID NO:
54;
(xix) primer pair 19 represented by a primer comprising SEQ ID NO:
56 and a primer comprising SEQ ID NO:
57;
(xx) primer pair 20 represented by a primer comprising SEQ ID NO:
59 and a primer comprising SEQ ID NO:
60;
(xxi) primer pair 21 represented by a primer comprising SEQ ID NO:
62 and a primer comprising SEQ ID NO:
63;
and
(xxii) primer pair 22 represented by a primer comprising SEQ ID NO:
65 and a primer comprising SEQ ID NO:
66;
and
(b) introgressing the allele of interest into Zea mays germplasm that lacks the allele.

67 more elements

A method for identifying a Zea mays plant comprising at least one allele associated with improved yield, the method comprising:

(a) genotyping at least one Zea mays plant with at least one nucleic acid marker selected from among SEQ ID NOs:
1, 4, 7, 10, 13, 16, 19, 22, 25, 28, 31, 34, 37, 40, 43, 46, 49, 52, 55, 58, 61, 64, and 111-173;
and
(b) selecting at least one Zea mays plant comprising an allele of at least one of the at least one nucleic acid marker that is associated with improved yield.

1 more element

An isolated and purified genetic marker associated with a yield trait in Zea mays, wherein the isolated and purified genetic marker:

(a) comprises a nucleotide sequence as set forth in any of SEQ ID NOs:
1-173, or the reverse complement thereof, or an informative fragment thereof;
and/or
(b) comprises a nucleotide sequence of an amplification product or an informative fragment thereof from a nucleic acid sample isolated from a Zea mays plant, wherein the amplification product is produced by amplifying a Zea mays nucleic acid using a pair of oligonucleotide primers selected from among SEQ ID NOs:
2 and 3;
SEQ ID NOs:
5 and 6;
SEQ ID SEQ ID NOs:
8 and 9;
SEQ ID NOs:
11 and 12;
SEQ ID NOs:
14 and 15;
SEQ ID NOs:
17 and 18;
SEQ ID NOs:
20 and 21;
SEQ ID NOs:
23 and 24;
SEQ ID NOs:
26 and 27;
or SEQ ID NOs:
29 and 30;
SEQ ID NOs:
32 and 33;
SEQ ID NOs:
35 and 36;
SEQ ID NOs:
38 and 39;
SEQ ID NOs:
41 and 42;
SEQ ID NOs:
44 and 45;
SEQ ID NOs:
47 and 48;
SEQ ID NOs:
50 and 51;
SEQ ID NOs:
53 and 54;
SEQ ID NOs:
56 and 57;
SEQ ID NOs:
59 and 60;
SEQ ID NOs:
62 and 63;
and SEQ ID NOs:
65 and 66.

44 more elements

Dependencies are inferred from the claim text.

Facts

Application: 12/536,147Filed 5 Aug 2009
Published: 10 Feb 2011
Term ends: Not estimatedThe source records don’t support an estimate.

Classification10 codes

A01H 1/045
Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection: using molecular markers
A01H 3/00
Processes for modifying phenotypes
A01H 5/10
Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy: seeds
A01H 6/4684
Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize: zea mays [maize]