Transcriptional repressor peptides and genes for the same
AssigneeNational Institute of Advanced Industrial Science and Technology
InventorsMasaru Takagi, Miho Ikeda
Life of this patent
Estimating the term…
Abstract
Provided is a peptide which is capable of repressing transcription in a plant and which can be used in the CRES-T (a simple and widely applicable means for repressing gene transcription), the peptide including an amino acid sequence represented by formula (I): X1-X2-Leu-Phe-Gly-Val-X3, where each of X1 and X3 independently represents an amino acid sequence consisting of any 1 to 10 amino acids, and X2 represents Lys or Arg. Also provided is a gene encoding the peptide.
Claims10
A peptide which is capable of repressing transcription in a plant, comprising an amino acid sequence represented by the following formula (I):
- X1-X2-Leu-Phe-Gly-Val-X3,
- where each of X1 and X3 independently represents an amino acid sequence consisting of any 1 to 10 amino acids, and X2 represents Lys or Arg.
Nucleic acid molecules encoding a chimeric protein which is capable of repressing transcription, comprising:
- first nucleic acid molecules encoding a peptide which is capable of repressing transcription in a plant;
- and
- second nucleic acid molecules encoding a transcription factor or its DNA-binding domain,
- the first nucleic acid molecules being linked in frame with the second nucleic acid molecules, and
- the peptide encoded by the first nucleic acid molecules including an amino acid sequence represented by the following formula (I):
- X1-X2-Leu-Phe-Gly-Val-X3,
- where each of X1 and X3 independently represents an amino acid sequence consisting of any 1 to 10 amino acids, and X2 represents Lys or Arg.
Method for producing a chimeric protein which is capable of repressing transcription, comprising the step of:
- linking (i) a peptide which is capable of repressing transcription in a plant to (ii) a transcription factor or its DNA-binding domain,
- the peptide including an amino acid sequence represented by the following formula (I):
- X1-X2-Leu-Phe-Gly-Val-X3,
- where each of X1 and X3 independently represents an amino acid sequence consisting of any 1 to 10 amino acids, and X2 represents Lys or Arg.
Dependencies are inferred from the claim text.