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US 2011/0035843 A1Published application
Novel eto1 genes and use of same for reduced ethylene and improved stress tolerance in plants
AssigneePioneer Hi-Bred International, Inc.
InventorsNicholas J. Bate, Xiaoming Bao
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Abstract
The invention provides isolated ethylene over-producer 1 (ETO1) nucleic acid molecules which are associated with ethylene production in plants and their encoded proteins. The present invention provides methods and compositions relating to altering ethylene production and abiotic stress response in plants. The invention further provides recombinant expression cassettes, host cells, transgenic plants, and antibody compositions.
Claims24
An isolated nucleic acid molecule comprising a member selected from the group consisting of:
- (a) a polynucleotide comprising a nucleotide sequence selected from the group consisting of the nucleotide sequences set forth in SEQ ID NO:
- 1, 3, 5, 7, and 9.
- (b) a polynucleotide having at least 80% sequence identity, as determined by the BLAST 2.0 algorithm under default parameters, to a nucleotide sequence selected from the group consisting of the nucleotide sequences set forth in SEQ ID NO:
- 1, 3, 5, 7, and 9, wherein the polynucleotide encodes a polypeptide comprising ETO1 activity;
- (c) a polynucleotide encoding a polypeptide comprising an amino acid selected from the selected from the group consisting the amino acid sequences set forth in SEQ ID NO:
- 2, 4, 6, 8, and 10;
- (d) a polynucleotide amplified from a nucleic acid library using primers which selectively hybridize, under stringent hybridization conditions, to loci within a polynucleotide selected from the group consisting the nucleotide sequences set forth in SEQ ID NO:
- 1, 3, 5, 7, and 9, wherein the polynucleotide encodes a polypeptide comprising ETO1 activity;
- (e) a polynucleotide which selectively hybridizes, under stringent hybridization conditions and a wash in 2× SSC at 50° C., to a polynucleotide selected from the group consisting of the nucleotide sequences set forth in SEQ ID NO:
- 1, 3, 5, 7, and 9, wherein the polynucleotide encodes a polypeptide comprising ETO1 activity;
- (f) a polynucleotide comprising a nucleotide sequence encoding an amino acid sequence having at least 80% sequence identity to an amino acid selected from the selected from the group consisting the amino acid sequences set forth in SEQ ID NO:
- 2, 4, 6, 8, and 10, wherein the polynucleotide encodes a polypeptide comprising ETO1 activity.
- (g) a polynucleotide which is the full-length complement of a polynucleotide of (a), (b), (c), (d), (e), or (f);
- and
- (h) a polynucleotide comprising at least 25 contiguous nucleotides from a polynucleotide of (a), (b), (c), (d), (e) ,or (f), wherein the polynucleotide encodes a polypeptide comprising ETO1 activity.
12 more elements
A method of modulating the ethylene production in a plant, comprising:
- (a) introducing into a plant cell a nucleotide construct comprising a polynucleotide which encodes an ETO1 protein operably linked to a promoter;
- (b) culturing the plant cell under plant cell growing conditions;
- and
- (c) regenerating a plant from said plant cell;
- wherein the ethylene production in said plant is modulated.
2 more elements
An isolated protein comprising a member selected from the group consisting of:
- (a) a polypeptide comprising an amino acid sequence selected from the group consisting of the amino acid sequence set forth in SEQ ID NO:
- 2, 4, 6, 8 and 10;
- (b) a polypeptide having at least 80% sequence identity to a polypeptide comprising an amino acid sequence selected from the group consisting of the amino acid sequences set forth in SEQ ID NO:
- 2, 4, 6, 8 and 10, wherein said sequence identity is determined using BLAST 2.0 under default parameters wherein the polypeptide comprises ETO1 activity;
- and
- (c) a polypeptide having at least 80% sequence identity to, and having at least one linear epitope in common with, a polypeptide comprising an amino acid sequence selected from the group consisting of the amino acid sequences set forth in SEQ ID NO:
- 2, 4, 6, 8 and 10, wherein said sequence identity is determined using BLAST 2.0 under default parameters, wherein the polypeptide comprises ETO1 activity;
- and
- (d) a polypeptide comprising at least 20 contiguous amino acids from a polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NO:
- 2, 4, 6, 8 and 10, wherein the polypeptide comprises ETO1 activity;
- and
- (e) a polypeptide encoded by a nucleotide sequence selected from the group consisting of the nucleotide sequences set forth in SEQ ID NO:
- 1, 3, 5, 7, and 9.
10 more elements
Dependencies are inferred from the claim text.