Nanocarrier based plant transfection and transduction
AssigneeAgriculture and Agri-Food Canada
InventorsFrancois Eudes, Archana Chugh
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Abstract
The present invention provides a novel method for the transduction and/or transfection of plant cells. Cell-penetrating peptides (CPPs) have been successfully employed as nanocarriers to deliver proteins and oligonucleotides to single plant cell microspores as well as multi-cellular zygotic embryos. The efficiency of CPP internalization and further delivery of a macromolecular cargo comprising a protein and/or an oligonucleotide can be enhanced by permeabilization of the zygotic embryos.
Claims31
A method for the delivery of a cargo moiety to plant tissues, organs, or cells, said method comprising exposing a plant cells to a complex comprising at least one cargo moiety linked to a carrier moiety.
A complex for mediating transport of an active substance into a plant cell, said complex comprising a cargo moiety linked to a carrier moiety wherein the carrier moiety can drive the complex into a plant cell.
Dependencies are inferred from the claim text.