Methods for Enhancing Stress Tolerance in Plants and Compositions Thereof
AssigneeMonsanto Technology LLC
InventorMary Fernandes
Life of this patent
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Abstract
Increased tolerance to abiotic stress in a plant is provided by introducing DNA expressing a cold shock protein, e.g. bacterial cold shock protein.
Claims26
-20. (canceled)
A drought tolerant transgenic plant that comprises in its genome a recombinant DNA molecule that expresses a cold shock protein, wherein said cold shock protein confers resistance to drought.
A transgenic propagule of a drought tolerant transgenic plant that comprises in it genome a recombinant DNA molecule that expresses a cold shock protein, wherein said cold shock protein confers resistance to drought.
A method of improving drought tolerance in a transgenic progeny plant comprising crossing a plant with a drought tolerant transgenic plant having a recombinant DNA expressing a cold shock protein wherein said cold shock protein confers resistance to drought and obtaining a transgenic progeny plant having said recombinant DNA.
A method of producing a drought tolerant transgenic plant comprising the steps of:
- a) inserting into the genome of plant cells a recombinant DNA molecule that comprises, in the 5′ to 3′ direction:
- a) a first DNA polynucleotide comprising a promoter that functions in plants and which is operably linked to;
- b) a second DNA polynucleotide that encodes a cold shock protein, operably linked to;
- c) a 3′ transcription termination DNA polynucleotide that functions as a polyadenylation sequence;
- b) obtaining transformed plant cell containing said recombinant DNA;
- c) regenerating transgenic plants from said plant cells;
- and d) selecting a transgenic plant having increased drought tolerance.
A method for increasing yield in a crop subject to water deficit during its growth, said method comprising planting seeds having a recombinant DNA expressing a cold shock protein wherein said cold shock protein confers resistance to drought and allowing said seeds to grow to mature plants under drought conditions.
An recombinant DNA molecule that comprises, in the 5′ to 3′ direction:
- a) a first DNA polynucleotide comprising a promoter that functions in plants and which is operably linked to;
- b) a second DNA polynucleotide that encodes a cold shock protein, operably linked to;
- c) a 3′ transcription termination DNA polynucleotide that functions as a polyadenylation sequence, wherein said second DNA polynucleotide encodes a protein that has an amino acid sequence of SEQ ID NO:
- 63 or SEQ ID NO:65.
A protein that has an amino acid sequence of SEQ ID NO:
- 63 or SEQ ID NO:65.
Dependencies are inferred from the claim text.