Codon optimized cftr

A method of producing hCFTR-encoding mRNA and hCFTR protein comprising:

• introducing a composition comprising a nucleic acid molecule comprising a sequence as shown in SEQ ID NO:
• 1 or 2 (DNA) or SEQ ID NO:
• 3 or 4 (RNA) into mammalian cells, whereby the cells express hCFTR-encoding mRNA and hCFTR protein.

A method of producing hCFTR-encoding mRNA and hCFTR protein comprising:

• introducing a composition comprising a nucleic acid molecule comprising a sequence as shown in SEQ ID NO:
• 1 or 2 (DNA) or SEQ ID NO:
• 3 or 4 (RNA) into human lung cells in a human Cystic Fibrosis patient via an aerosol, wherein the nucleic acid molecule is compacted in particles with a polycation, wherein the particles are unimolecular with respect to nucleic acid, whereby the cells express hCFTR-encoding mRNA and hCFTR protein.

A method to increase the expression of an mRNA or protein from a cDNA molecule, comprising:

• inspecting the cDNA molecule to ascertain the presence of a premature transcription termination signal;
• eliminating the premature transcription termination signal of the cDNA molecule without altering its encoded amino acid sequence;
• introducing the cDNA into a cell whereby it is expressed.