Process for Isolation and Purification of Geldanamycin

A process for the purification of geldanamycin, said process comprising steps of

• a. extraction of a fermentation broth containing geldanamycin with an organic solvent or a mixture of organic solvents;
• b. adsorption of extracted product of step (a) on a solid support;
• c. washing of the product-containing solid support of step (b) with an organic solvent or a mixture of organic solvents or a mixture of an organic solvent and water;
• d. further washing the product-containing solid support from step (c) with an organic solvent or mixture of organic solvents to cause product elution;
• e. crystallizing the eluted product from step (d) followed by filtration;
• f. purification of the filtered solids from step (e) by making a suspension in an organic solvent or mixture of organic solvents and filtration;
• g. optional repetition of step (f) and
• h. drying