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AssigneesJapan Advanced Institute of Science and Technology, Japan Science and Technology Agency
InventorsKenzo Fujimoto, Yoshinaga Yoshimura
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Disclosed is a method for the purification and collection of a nucleic acid comprising a specific nucleotide sequence, which can be carried out within an extremely short period and can achieve both high sequence-specificity and a high collection rate. Specifically disclosed is a method for the purification of a target nucleic acid comprising a specific nucleotide sequence and contained in a nucleic acid mixture. The method comprises the steps of: hybridizing a photo-ligating nucleic acid having a group represented by formula (I) as abase moiety with the target nucleic acid to form a hybrid; irradiating the hybrid of the photo-ligating nucleic acid and the target nucleic acid with light to cause the photo-ligation of the hybrid; removing any un-photo-ligated nucleic acid by washing; and irradiating the hybrid of the photo-ligating nucleic acid and the target nucleic acid with light to cause the photo-cleavage of the hybrid.
A method of purifying a target nucleic acid having a particular base sequence that is contained in a nucleic acid mixture, the method comprising:
A photo-ligating nucleic acid (wherein the nucleic acid includes a nucleic acid and a peptide nucleic acid) having a labeling site and having a group represented by the following formula (I) as a base moiety:
A photo-ligating nucleic acid (wherein the nucleic acid includes a nucleic acid and a peptide nucleic acid) immobilized onto a support and having a group represented by the following formula (I) as a base moiety:
Dependencies are inferred from the claim text.