Oligonucleotide sequence formula for labeling olignucleotide probes and proteins for in-situ analysis

A synthetic oligonucleotide probe having a 5′ end and a 3′ end and comprising:

• a first domain consisting of the sequence 5′-(AAAATAG)n-3′ wherein “n” is at least four, wherein the first domain does not hybridize with human nucleic acids under standard stringency conditions, wherein the first domain is detectably labeled, and wherein the 5′ end of the probe consists of the first domain;
• and
• a second domain comprising a target sequence capable of hybridizing to a DNA or RNA sequence, wherein the second domain is not detectably labeled, the beginning of the second domain starting at the 3′ end of the first domain and the end of the second domain being the 3′ end of the probe, wherein the first domain and second domain comprise a single polynucleotide molecule.

A synthetic oligonucleotide probe having a 5′ end and a 3′ end and comprising:

• a first domain consisting of the sequence 5′-(AAAATAG)n-3′ wherein “n” is at least four, wherein the first domain does not hybridize with human nucleic acids under standard stringency conditions, wherein the first domain is detectably labeled, and wherein the 3′ end of the probe consists of the first domain;
• and
• a second domain comprising a target sequence capable of hybridizing to a DNA or RNA sequence, wherein the second domain is not detectably labeled, the beginning of the second domain starting at the 5′ end of the probe and the end of the second domain being at the 5′ end of the first domain, wherein the first domain and second domain comprise a single polynucleotide molecule.