Assay for imidazolinone resistance mutations in brassica species
AssigneeAdvanta Canada, Inc.
InventorsStephen Barnes, Sigrid Vanstraelen
Life of this patent
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Abstract
The invention provides methods and oligonucleotide primers for assaying Brassica napus plants for the presence or absence of mutations that confer resistance to imidazolinone herbicides. Specifically, the methods and primers of the invention are useful for detecting the PM1 mutation of the B. napus AHAS1 gene and the PM2 mutation of the B. napus AHAS3 gene.
Claims2
An amplification primer selected from the group consisting of an oligonucleotide having a sequence as set forth in SEQ ID NO:9;
- an oligonucleotide having a sequence as set forth in SEQ ID NO:10;
- an oligonucleotide having a sequence as set forth in SEQ ID NO:11;
- an oligonucleotide having a sequence as set forth in SEQ ID NO:12;
- an oligonucleotide having a sequence as set forth in SEQ ID NO:13;
- an oligonucleotide having a sequence as set forth in SEQ ID NO:14;
- an oligonucleotide having a sequence as set forth in SEQ ID NO:15;
- an oligonucleotide having a sequence as set forth in SEQ ID NO:16;
- an oligonucleotide having a sequence as set forth in SEQ ID NO:17;
- and an oligonucleotide having a sequence as set forth in SEQ ID NO:18.
A nucleic acid selected from the group consisting of a nucleic acid having a sequence as set forth from nucleotide 96 to nucleotide 2330 of SEQ ID NO:19;
- a nucleic acid having a sequence as set forth from nucleotide 1817 to nucleotide 2063 of SEQ ID NO:1;
- a nucleic acid having a sequence as set forth from nucleotide 1735 to nucleotide 1980 of SEQ ID NO:2;
- a nucleic acid having a sequence as set forth from nucleotide 1809 to nucleotide 2054 of SEQ ID NO:3;
- a nucleic acid having a sequence as set forth from nucleotide 1720 to nucleotide 1966 of SEQ ID NO:4;
- a nucleic acid having a sequence as set forth from nucleotide 64 to nucleotide 2310 of SEQ ID NO:20;
- a nucleic acid having a sequence as set forth from nucleotide 1383 to nucleotide 1770 of SEQ ID NO:5;
- a nucleic acid having a sequence as set forth from nucleotide 1518 to nucleotide 1905 of SEQ ID NO:6;
- a nucleic acid having a sequence as set forth from nucleotide 1352 to nucleotide 1739 of SEQ ID NO:7;
- a nucleic acid having a sequence as set forth from nucleotide 1308 to nucleotide 1695 of SEQ ID NO:8;
- a nucleic acid having a sequence as set forth from nucleotide 1560 to nucleotide 1770 of SEQ ID NO:5;
- a nucleic acid having a sequence as set forth from nucleotide 1695 to nucleotide 1905 of SEQ ID NO:6;
- a nucleic acid having a sequence as set forth from nucleotide 1529 to nucleotide 1739 of SEQ ID NO:7;
- and a nucleic acid having a sequence as set forth from nucleotide 1485 to nucleotide 1695 of SEQ ID NO:8.
Dependencies are inferred from the claim text.