Systems for Expression of Heterologous Proteins in M. Capsulatus

A method for producing a desired protein in a bacterial host cell, said method comprising transforming a bacterial host cell with a recombinant vector comprising a first nucleotide sequence Seq. ID no. 4 or sequences homologous thereto, excluding sequences comprising the N-terminal sequence present in Seq. ID no 3 but not in Seq. ID no 4, and said vector further comprising a nucleotide sequence encoding said protein, said further nucleotide sequence being operably linked in frame to said first nucleotide sequence, and culturing said transformed host cell in a suitable medium under conditions allowing expression of said protein.

A protein capable of being exposed on the surface of a methanotrophic bacterium, wherein the protein is encoded for by Seq. ID no. 4 or sequences homologous thereto, excluding sequences comprising the N-terminal sequence present in Seq. ID no 3 but not in Seq. ID no 4.

A fusion protein comprising a protein or peptide sequence encoded for by a nucleotide sequence Seq. ID no. 4 or sequences homologous thereto, excluding sequences comprising the N-terminal sequence present in Seq. ID no 3 but not in Seq. ID no 4, and a desired protein or peptide, capable of being translocated.