Controlling the potential of primate neural stem cells by regulating pax6
AssigneeWisconsin Alumni Research Foundation
InventorsSU-CHUN ZHANG, XIAOQING ZHANG
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Abstract
A transcription factor both necessary and sufficient for human neuroectoderm specification, Pax6, as well as applications thereof, is disclosed.
Claims20
A primate primitive neural stem cell (primate pNSC) wherein the cell overexpresses Pax6.
A method of creating a population of primate pNSCs from primate embryonic stem cells (ESCs) or induced pluripotent stem cells (iPSCs) comprising the step of:
- overexpressing Pax6 within a population of ESCs or iPSCs, wherein the expression level of Pax6 is sufficient to suppress the expression of Sox1 and Oct4.
A method of creating a population of primate regional neural stem cells comprising the steps of:
- (a) overexpressing Pax6 within a population of primate ESCs or iPSCs wherein the expression level of Pax6 is sufficient to suppress the expression of Sox1 and Oct4;
- and
- (b) suppressing Pax6 expression and differentiating the cells of step (b) into regional neural stem cells.
A method of creating a population of primate pNSCs from primate regional neural stem cells comprising the step of:
- overexpressing Pax6 within a population of primate regional neural stem or progenitor cells wherein the expression level of Pax6 is sufficient to reprogram the cells to the primate pNSC stage.
A method of treating a patient with brain tumor or overgrowth of cell transplants by suppressing Pax6 expression comprising the steps of inhibiting Pax6 expression in the tumor cells or the overgrown cell transplants.
Dependencies are inferred from the claim text.