Positive controls for expression modulating experiments
AssigneeQiagen GmbH
InventorsPeter Hahn, Jörg Dennig, Wolfgand Bielke, Jie Kang
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Abstract
The invention pertains to the use of an apoptosis inducing combination of at least a. a first expression modulating compound silencing the expression of at least a first target gene involved in apoptosis and b. a second expression modulating compound silencing the expression of at least a second target gene involved in apoptosis as a positive control in expression modulating assays. Also provided are suitable methods, kits and compositions.
Claims20
A method for performing an expression modulating assay, comprising:
- performing an expression modulating assay, wherein
- an apoptosis inducing combination of at least
- a) a first expression modulating compound silencing the expression of at least a first target gene involved in apoptosis, wherein said first target gene is Plk1 and
- b) a second expression modulating compound silencing the expression of at least a second target gene involved in apoptosis, wherein said second target gene is ubiquitin,
- is used as a positive control in the expression modulating assay.
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A method for performing an expression modulating analysis or assay, wherein an apoptosis inducing combination of at least
- a) a first expression modulating compound silencing the expression of at least a first target gene involved in apoptosis, wherein the first target gene is Plk1, and
- b) a second expression modulating compound silencing the expression of at least a second target gene involved in apoptosis, wherein said second target gene is ubiquitin,
- is introduced into cells to induce apoptosis in the cells.
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11-12. (canceled)
An RNAi inducing composition inducing apoptosis comprising:
- a) an RNAi inducing compound targeting the transcript of the ubiquitin genes;
- and
- b) an RNAi inducing compound targeting the transcript of the Plk1 gene.
15-17. (canceled)
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Dependencies are inferred from the claim text.