Compositions and methods for analyzing bacterial adherence and anti-adherence to mucus, epithelial cells and other cells
AssigneeAlltech, Inc.
InventorsKarl A. Dawson, Colm Moran, Juha Apajalahti, Marko Lauraeus
Life of this patent
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Abstract
The present invention generally relates to methods for detecting, identifying, and measuring bacterial adherence to mucus and epithelial cells. In particular, the present invention provides assays for detecting and identifying the presence or absence of bacterial adherence to mucus (epithelial cells (e.g., present in the intestines), or other portion of an animal where bacteria may be present, and methods for identifying and characterizing (e.g., the efficacy of) modulators of bacterial adherence to mucus and epithelial cells, or other portion of the animal where bacteria may be present.
Claims57
A kit comprising a non-radioactive enzyme-linked immunosorbent assay (ELISA) for the assay of bacterial adherence and anti-adherence with mucus and/or epithelial cells comprising a solid support having mucus and/or epithelial cells coated thereon, a sample comprising bacteria, a primary antibody specific for said bacteria, and a detectably labeled secondary antibody specific for said primary antibody bound to said bacteria.
A method for measuring adherence and anti-adherence between bacteria and mucus and/or epithelial cells comprising
- a) providing
- i) a sample comprising bacteria;
- and
- ii) mucus and/or epithelial, or other cells;
- and
- b) combining said sample comprising bacteria and said mucus and/or epithelial cells within a non-radioactive colorimetric assay under conditions such that adherence and anti-adherence between said bacteria and said mucus and/or epithelial cells is measured.
A method for identifying an agent that modulates adherence between bacteria and mucus and/or epithelial cells, comprising
- a) providing
- i) a sample comprising bacteria;
- ii) mucus and/or epithelial cells;
- and
- iii) an agent;
- and
- b) combining said sample comprising bacteria, said mucus and/or epithelial cells, and said agent within a non-radioactive colorimetric assay under conditions such that adherence between said bacteria and said mucus and/or epithelial cells is measured;
- c) comparing said bacterial adherence in the presence and absence of said agent;
- and
- d) identifying said agent as a modulator of adherence between said bacteria and said mucus and/or epithelial cells if said measured adherence is higher or lower than adherence between said bacteria and said mucus and/or epithelialcells in the absence of said agent.
A composition comprising an agent, wherein said agent is a modulator of bacterial adherence with mucus and/or epithelial cells, wherein said agent is identified through a process comprising:
- a) providing
- i) a sample comprising bacteria;
- ii) mucus and/or epithelial cells;
- and
- iii) an agent;
- and
- b) combining said sample comprising bacteria, said mucus and/or epithelial cells, and said agent within a non-radioactive colorimetric assay under conditions such that adherence between said bacteria and said mucus and/or epithelial cells is measured;
- c) comparing said bacterial adherence in the presence and absence of said agent;
- and
- d) identifying said agent as a modulator of adherence between said bacteria and said mucus and/or epithelial cells if said measured adherence is higher or lower than adherence between said bacteria and said mucus and/or epithelial cells in the absence of said agent.
Dependencies are inferred from the claim text.