US 2011/0034370 A1 · BIS-MET HISTONES

Abstract

The present invention provides a nucleic acid molecule which encodes a polypeptide consisting of two methionine residues as the first and second N-terminal amino acid residues linked via a peptide bond to a mature eukaryotic histone. The present invention furthermore relates to a vector containing said nucleic acid molecule, a host transformed with said vector, polypeptides encoded by the nucleic acid molecule and pharmaceutical and diagnostic compositions. The present invention also relates to the use of the nucleic acid molecule, vectors, hosts and the polypeptide of the invention for the preparation of a composition for the treatment of diseases. Furthermore, the present invention relates to a method of testing for the presence of the nucleic acid molecule or the polypeptide in a sample and to a kit.

Claims21

Dependent claims

An isolated nucleic acid molecule which

a) encodes a polypeptide comprising
(aa) two methionine residues as the first and second N-terminal amino acid residues linked via a peptide bond to
(ab) a mature eukaryotic histone;
b) encodes a polypeptide comprising
(ba) two methionine residues as the first and second N-terminal amino acid residues linked via a peptide bond to
(bb) a mature eukaryotic polypeptide having at least 80% sequence identity to the mature eukaryotic histone of (a) and essentially retaining its biological activity;
or
c) hybridizes under stringent conditions to the complementary strand of a nucleic acid molecule encoding the polypeptide of (a) or (b), wherein said nucleic acid molecule encodes a polypeptide having at least the two N-terminal methionine residues and essentially retains the biological activity of the polypeptide of (a) or (b).

5 more elements

Dependencies are inferred from the claim text.

Bis-met histones

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Bis-met histones

Life of this patent

Abstract

Claims21