CANINE iPS CELLS AND METHOD OF PRODUCING SAME
AssigneeKyoto University
InventorsTatsuo NAKAMURA, Hidenori SHIMADA
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Abstract
Provided are a method of producing canine iPS cells, comprising (a) the step of bringing into contact with each other a canine somatic cell and a nuclear reprogramming factor, and (b) the step of culturing the cell in a medium containing at least one substance selected from the group consisting of a mitogen-activated protein kinase kinase inhibitor, an activin receptor-like kinase inhibitor, a glycogen synthase kinase inhibitor, a L-type calcium channel agonist and a DNA methylation inhibitor, and a leukemia inhibitory factor, and canine iPS cells that can be obtained by the method.
Claims16
A method of producing a canine iPS cell, comprising the steps (a) and (b) below:
- (a) the step of bringing into contact with each other a canine somatic cell and a nuclear reprogramming factor,
- (b) the step of culturing the cell in a medium containing one or more substance(s) selected from the group consisting of a mitogen-activated protein kinase kinase inhibitor, an activin receptor-like kinase inhibitor, a glycogen synthase kinase inhibitor, a L-type calcium channel agonist and a DNA methylation inhibitor, and a leukemia inhibitory factor.
A canine pluripotent stem cell of somatic cell derivation possessing pluripotency and a potential for self-replication.
Dependencies are inferred from the claim text.