Rationally-designed meganucleases with recognition sequences found in dnase hypersensitive regions of the human genome
AssigneePrecision BioSciences, Inc.
InventorsDerek Jantz, Michael G. Nicholson, James J. Smith
Life of this patent
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Abstract
Rationally-designed LAGLIDADG meganucleases and methods of making such meganucleases are provided. In addition, methods are provided for using the meganucleases to generate recombinant cells and organisms having a desired DNA sequence inserted into a limited number of loci within the genome, as well as methods of gene therapy, for treatment of pathogenic infections, and for in vitro applications in diagnostics and research.
Claims10
1-49. (canceled)
A recombinant meganuclease having altered cleavage specificity relative to a wild-type I-CreI meganuclease of SEQ ID NO:1, comprising a polypeptide having at least 85% sequence similarity to residues 2-153 of the I-CreI meganuclease of SEQ ID NO:
- 1;
- and
- having specificity for a recognition sequence half-site which differs by at least one base pair from a half-site within an I-CreI meganuclease recognition sequence selected from the group consisting of SEQ ID NO:
- 2, SEQ ID NO;
- 3, SEQ ID NO:
- 4 and SEQ ID NO:
- 5;
- wherein the recognition sequence half-site is found in a DNaseI hypersensitive region of the human genome and is selected from the group consisting of SEQ ID NO:
- 59, 46, 71, 81, 62, 37-45, 47-58, 60-61, 63-70, 72-80 and 82-87.
Dependencies are inferred from the claim text.