Transcriptome Transfer Produces Cellular Phenotype Conversion
AssigneeThe Trustees of the University of Pennsylvania
InventorsJames Eberwine, Jai-Yoon Sul, Chia-Wen Wu, Fanyi Zeng, Junhyong Kim
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Abstract
The present invention includes methods for effecting phenotype conversion in a cell by transfecting the cell with phenotype-converting nucleic acid. Expression of the nucleic acids results in a phenotype conversion in the transfected cell. Preferably the phenotype-converting nucleic acid is a transcriptome, and more preferably an mRNA transcriptome.
Claims26
A method of effecting phenotype conversion in a cell, said method comprising transfecting phenotype-converting nucleic acid of a first cell having a phenotype into a second cell having a phenotype, wherein the phenotype of said first cell is different from the phenotype of said second cell, and wherein said transfected phenotype-converting nucleic acid causes the phenotype of said second cell to change to the phenotype of said first cell.
A method of preparing a cell enriched for the presence of a second messenger system pathway, said method comprising transfecting a heterogenous collection of mRNAs encoding the components of a second messenger system pathway into a cell, wherein expression of said transfected heterogenous collection of mRNAs enriches the presence of the second messenger system pathway.
A method of deleting a gene of interest in a cell, said method comprising transfecting an mRNA encoding an enzyme capable of sequence specific excision into a cell, said cell comprising chromosomal material that comprises a gene of interest that is flanked by recognition sequences for the enzyme,
- wherein expression of said mRNA provides the enzyme capable of sequence specific excision, and wherein said enzyme that binds to the enzyme recognition sequences and excises the gene of interest.
Dependencies are inferred from the claim text.