Methods and compositions for growth of cells and embryonic tissue on a synthetic polymer matrix
AssigneeThe Regents of the University of Michigan
InventorsGary D. Smith, Joerg Lahann, Himabindu Nandivada, Thomas Eyster, Luis Villa
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Abstract
The present invention provides methods and compositions for establishing and maintaining growth of cells and embryonic tissue on a synthetic polymer matrix. For example, the present invention provides synthetic growth matrices for stem cells, gametes, mature differentiated cells, and embryonic tissue (e.g., blastomeres, embryos, and embryoid bodies). In certain embodiments, the cells are capable of going through multiple passages while remaining in an undifferentiated state as a result of the synthetic polymer matrix.
Claims17
A composition for growth and maintenance of cells or embryonic tissue comprising:
- a synthetic polymer matrix and a culture medium, wherein said synthetic polymer matrix is selected from the group consisting of:
- i) a first polymer which comprises a zwitterionic group;
- ii) a co-polymer comprising poly[2-(methacryloyloxy)ethyl dimethyl-(3-sulfopropyl)ammonium hydroxide] (PMEDSAH) and poly(ethylene glycol methacrylate) (PPEGMA);
- iii) a copolymer comprising PMEDSAH and poly(2-(methacryloyloxy)ethyl)trimethylammonium chloride (PMETAC);
- and
- iv) a copolymer comprising 2-hydroxyethyl methacrylate (HEMA) and PMEDSAH.
A method for culturing cells or embryonic tissue comprising
- a) applying cells to a substrate, wherein said substrate comprises a culture medium and a synthetic polymer matrix, wherein said synthetic polymer matrix is selected from the group consisting of:
- i) a first polymer which comprises a zwitterionic group;
- ii) a co-polymer of poly[2-(methacryloyloxy)ethyl dimethyl-(3-sulfopropyl)ammonium hydroxide] (PMEDSAH) and poly(ethylene glycol methacrylate) (PPEGMA);
- iii) a copolymer of PMEDSAH and poly(2-(methacryloyloxy)ethyl)trimethylammonium chloride (PMETAC);
- and
- iv) a copolymer of 2-hydroxyethyl methacrylate (HEMA) and PMEDSAH;
- and b) growing said cells or said embryonic tissue on said substrate.
Dependencies are inferred from the claim text.