Production Of Viral Vectors
AssigneeThe Regents of the University of Michigan
InventorsJeffrey S. Chamberlain, Dennis J. Hartigan-O'Connor
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Abstract
The present invention relates to methods and compositions for the production of viral vectors. In particular, the present invention provides methods and compositions for faster, higher titer and higher purity production of viral vectors (e.g. adenoviral vectors). In some embodiments, the present invention provides gutted and helper viruses with identical or similar termini. In other embodiments, the present invention provides terminal protein linked adenoviral DNA. In certain embodiments, the present invention provides template extended adenoviral DNA.
Claims20
A method for producing helper-dependent viral vectors comprising:
- a) providing;
- i) helper-dependent viral DNA comprising a first origin of replication,
- ii) helper viral DNA comprising a second origin of replication, wherein said second origin of replication has similar activity level in a replication assay as said first origin of replication, and
- iii) target cells;
- and
- b) transfecting said target cells with said helper-dependent viral DNA and said helper viral DNA under conditions such that helper-dependent viral vectors are produced.
A host cell comprising;
- a) helper-dependent viral DNA comprising a first origin of replication, and
- b) helper viral DNA comprising a second origin of replication, wherein said second origin of replication has a similar activity level in a replication assay as said first origin of replication.
A method for producing helper-dependent viral vectors comprising:
- a) providing;
- i) helper-dependent viral DNA comprising an origin of replication linked to a replication-promoting agent, and
- ii) target cells;
- and
- b) transfecting said target cells with said helper-dependent viral DNA under conditions such that helper-dependent viral vectors are produced.
Dependencies are inferred from the claim text.