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US 2011/0033903 A1Published application
Isolated population of plant single cells and method of preparing same
AssigneeUnhwa Corporation
InventorYoung Woo Jin
Life of this patent
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Abstract
This invention is a method of minimizing the variation of cell growth and production through homogeneous cell line development. To be more specific, it is the method of isolating and proliferating single cell clone from cambium to promote the stability of the plant-derived biologically active substances production by solving the problems of decrease in cell growth and the productivity during the long term culture.
Claims15
A method for preparing an isolated population of cells, the method comprising:
- (a) providing a tissue from a plant;
- (b) obtaining from the plant tissue a tissue containing cambium;
- (c) culturing in a medium the tissue containing cambium;
- and
- (d) isolating the population of cells from the cambium,
- wherein the isolated cells are innately undifferentiated.
3 more elements
The method according to claim 2, wherein in the step (c), the medium includes auxin.
Dependencies are inferred from the claim text.