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AssigneeInstitut Biokhimii I Genetiki Ufimskogo Nauchnogo Tsentra Ran
InventorsDmitry Alekseevich Chemeris, Aleksei Viktorovich Chemeris, Eduard Gavisovich Magdanov, Ravil Rinatovich Garafutdinov, Vener Absatarovich Vakhitov, Said Fedorovich Urmancheev, Yury Anatolievich Lebedev
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The invention relates to a method for rapid detection of specific nucleic acid fragments with the aid of a PCR, consisting in amplifying target products in a special DNA thermocycler, provided with a special reaction thermal unit, which makes it possible to obtain, in reaction vessels in the form of standard polypropylene test tubes, a sloping temperature gradient directed at an angle to the direction of gravity. The amplification time is of 1-5 minutes. The inventive method can be recommended for DNA diagnostics, also field conditions, in medicine, veterinary sciences, in sanitary and epidemiological studies for detecting agents of dangerous infections, including potential bio-terrorist attacks, in criminalistics for identifying criminals in the food industry for detecting food products from genetically modified organisms, for testing raw material quality etc.
A method for amplification of specific fragments of nucleic acids with the aid of a polymerase chain reaction, characterized in that due to the effect of accelerated convection occurring as a result of a force of buoyancy caused by a sloping temperature gradient applied to a reaction vessel and oriented at an angle to the direction of action of gravity, cyclic temperature changes that are necessary for multiple sequential implementation of steps of denaturation, annealing and elongation in the reaction mixture due to constant, directed displacement of liquid layers very rapidly occur.
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