Method For Producing Human Antibodies With Properties Of Agonist, Antagonist, or Inverse Agonist
OwnerLi-Te CHIN, Shu-Ching Hsuinventors
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Abstract
A method for obtaining agonist, antagonist and inverse agonist, to a given physiological receptor is disclosed. For the method, use is made of in silico design synthetic immunogens, which are caused to act in vitro on human lymphocyte-containing cell populations. A preferred receptor is human CD152, particularly the regions of CDR1, CDR2 and CDR3 that elicit antibodies serving as antagonist, inverse agonist and agonist, respectively. Also provided is a method in the treatment of human peripheral lymphocytes for use in the screening of CD152 ligands that yield pharmacological effects.
Claims4
A method for producing human antibodies with properties of agonist, antagonist and/or inverse agonist to a receptor, comprising following steps:
- (a) defining peptide fragments resembling respective extracelluar domains of a receptor by the use of in silica characteristics of the respective extracellular domains of the receptor;
- (b) optionally defining amino acid sequences of coupled-fragments having the peptide fragments and a T-helper epitope by reassembling or coupling the peptide fragments and the T-helper in silica;
- wherein at least one T-helper is coupled with a peptide fragment;
- (c) preparing immunogens having amino acid sequences of the coupled-fragments;
- (d) stimulating human lymphocytes with the immunogens in vitro;
- (e) identifying and optionally screening the human lymphocytes that produce antibodies able to recognize the receptor;
- and
- (f) collecting the antibodies.
Dependencies are inferred from the claim text.